Question

It seems that the overview camera and objective camera are not properly aligned on my uScopeHXII. When I click on a part of the specimen that appears in the overview image, the corresponding part of the specimen does not always appear under the objective. Sometimes, I must move the objective one or two fields to find the matching part of the specimen.

Is there some calibration that needs to be performed from time to time?

Answer

There is no calibration that must be performed to adjust the targeting between the overview image and the objective.

The behavior you describe is expected of the uScope depending on the objective magnification.

The overview image is a relatively low-resolution picture of the slide. In cases of high magnification (40x or 60x objective) a few pixels of the overview image are equivalent to the whole image captured by the objective.

While the mapping between the overview image and objective may not be precisely one-to-one, it should be very close (as shown in the following overview and objective images).

Overview Image

Objective Image

Note
While unlikely, it is possible that the objective position has become out of sync with the overview image. You may cycle power to the uScope (by turning it off and back on) to re-synchronize the objective position.