uScope MXII

Stitching Issues with 40x and 60x Objectives
uScope MXII

Information in this article applies to:

  • uScope Navigator V4.0 and Later

Article ID: MXB1125 — Created: 3 Jan 2018 — Reviewed: 2 Feb 2018


On occasion, scanned regions do not combine (stitch) accurately for the 40x and 60x models of the uScopeMXII and for the 40x model of the uScopeHXII. This problem is illustrated in the image as indicated.


In some cases, the default overlap settings may not be adequate to stitch adjacent fields. Higher objective powers magnify the image and reduce the area included in a single field. This smaller, magnified area may not contain enough image content to stitch with adjacent fields. In such cases, a larger overlapped area may be required to render more pleasing stitched results.


In most cases, the default Overlap for Stitching setting (which is a default of 128 pixels) is more than adequate for combining adjacent fields. However, the uScope Navigator application also allows you to specify the number of pixels of overlap to use.

From the Device Settings — Scan Options dialog, select Custom for the Overlap Setting to manually specify the X and Y overlap values.

For 40x objectives...

X and Y settings of 144 pixels should be sufficient for most tissue specimens.

For 60x objectives...

X and Y settings of 144 pixels should be sufficient for most tissue specimens.

To speed-up scans...

You may wish to reduce the overlap to 96 or even 64 pixels. Shrinking the amount of overlapped area requires fewer fields for the scanned region. You can also experiment with different values for the X and Y overlap settings.

For more information about overlap settings...

Refer to Changing Image Overlap Settings.

Rate This Article
Contact Microscopes International or speak with your local distributor.
Copyright © 2018 Microscopes International, LLC. All rights reserved.