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uScope Navigator (Device Settings)

Recalibrating the Overview Camera
uScope Navigator (Device Settings)

Article ID: NDS1008
Created: 1 Jan 2017
Reviewed: 31 Mar 2017

Information in this article applies to:

  • uScope Navigator (All Versions)

Symptom

Overview Image with Poor Flat-FieldYou may observe that the overview image is not ideal and may have one or more of the following issues:

  • The image is brighter on one side than it is on the other.
  • There are artifacts in the image due to dirt or dust on the diffuser.
  • Color balance is biased toward a single color.

The image shown illustrates a poor overview image.

Cause

There may be multiple causes for this problem:

  1. The overview camera is out of color calibration.
  2. The flat-field image for the overview camera needs to be re-acquired.
  3. The filter applied to the overview camera may not include a flat-field correction.

Fortunately, these issues are all easy to solve by recalibrating the overview camera and verifying the applied filter.

Resolution

Device - Objective Camera SettingsTo correct the overview image issues:

  1. Open the Device Settings from the uScope Control Panel.
  2. Select the Overview Camera Settings tab.
  3. Click the Auto Calibrate button.
  4. If there is a slide in the uScope, remove it at this time and click OK to begin calibrating.
  5. A dialog shows the progress of the calibration.

    Progress Dialog
  6. After calibration completes, make sure a filter that includes flat-field correction is selected. The uScope comes with an overview filter named FF (for flat-field) that is selected by default.
  7. Click the Save and Exit button to save the changes and exit Device Settings.

Overview Image with Good Color Balance and Flat-FieldAt this point, you should load a slide into the uScope and review the overview image. It should appear as shown.

It is important to note that you may need to re-calibrate the overview camera from time-to-time. Once the camera is calibrated and color corrected, you should only need to re-calibrate if there have been changes to your computer's display settings or if dust has found its way onto the overview diffuser.

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