You may observe that the image captured by the objective camera is not ideal and may have one or more of the following issues:
- The image is brighter on one side than it is on the other.
- Color balance is biased toward a single color.
- There are artifacts in the image due to dirt or dust. In this case, you should contact your distributor or Microscopes International to have your uScope thoroughly cleaned. The uScope light path is a closed path and should rarely require cleaning.
There may be multiple causes for these types of problem:
- The objective camera is out of color calibration.
- The flat-field image for the objective camera needs to be re-acquired.
- The filter applied to the objective camera may not include a flat-field correction or may have an exaggerated color correction.
Fortunately, these issues are all easy to solve by recalibrating the objective camera and verifying the applied filter.
To correct the objective image issues:
- Open the Device Settings from the uScope Control Panel.
- Select the Objective Camera Settings tab.
- Click the Auto Calibrate button.
- A dialog shows the progress of the calibration.
- After calibration completes, make sure a filter that includes flat-field correction is selected. The uScope comes with an objective filter named FF (for flat-field) that is selected by default.
- Click the Save and Exit button to save the changes and exit Device Settings.
At this point, you should load a slide into the uScope and review the objective image.
It is important to note that you may need to re-calibrate the objective camera from time-to-time. Once the camera is calibrated and color corrected, you should rarely need to re-calibrate.
Refer to the following knowledge base article to learn more about flat-field correction.