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uScope Navigator (Filter Manager)

What is Flat-Field Correction?
uScope Navigator (Filter Manager)

Article ID: NFL1033
Created: 2 Apr 2017
Reviewed: 4 Apr 2017

Information in this article applies to:

  • uScope Navigator (All Versions)

Question

What is flat-field correction?

Answer

Flat field correction is a technique that improves image quality by removing artifacts that are caused by variations in the pixel-to-pixel sensitivity of the camera (image sensor) and/or illuminator. Additionally, flat field correction can remove distortions in the optical path (including small dirt particles on the image sensor). It is a standard calibration procedure in contemporary digital cameras.

  • For the overview camera, flat field correction uses the flat field image that is created when you recalibrate.
  • For the objective camera, flat field correction uses the flat field image that is created when you recalibrate and when the uScope self-calibrates before each scan.

Flat field correction eliminates or reduces gradient effects of the illuminator and objective as well as optical distortions like dirt on the image sensor. It will not eliminate the effects of dirt or debris on the slide or cover slip.

The following two images illustrate the effects of flat-field correction.

Image Before Flat-Field Correction
Image Before Flat-Field Correction

Image After Flat-Field Correction
Image After Flat-Field Correction

Note
The flat-field correction filter is available only for overview and slide camera filter sequences. It may be selected as the first filter in a sequence only.

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