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Filter Manager Flat-Field Correction Problems
Filter Manager

Information in this article applies to:

  • uScope Navigator V4.5 and Later

Article ID: NFL1185 — Created: 21 Aug 2019 — Reviewed: 22 Aug 2019

Symptom

Flat field correction does not always produce images with flat backgrounds. In some cases, images processed with the flat field correction filter appear worse than unfiltered images.

Cause

An inconsistency was discovered in the way images were processed when applying flat field correction. In some, but not all, cases the flat field filter created images with exaggerated background corrections. The problem had to do with calculating color average (mean) versus median values for calibration images.

Resolution

There are a number of filter tweaks that can be used to improve image flatness. However, correcting the calculations mentioned above provides the best flat field correction possible.

Status

This correction is available in uScope Navigator v4.5 and later which may be downloaded from the Microscopes International web site.

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