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Article ID: NGN1206 — Created: 21 Aug 2019 — Reviewed: 22 Aug 2019
uScope Navigator v4.5 adds support for EDF (Extended Depth of Field) image composition. This support is composed of several processes:
These tasks are described in details in the following sections.
Creating EDF images requires an additional software license. Please contact our sales department for more information.
Multi-Layer Scanning or Image Stacking involves taking several images at each scan field position at different focus points. Different power objectives have different depth of field (the thickness of the specimen that is in focus without moving the objective). Higher power objectives have narrower depth of field (less of the specimen is in focus) while lower power objectives have wider depth of field (more of the specimen is in focus).
In any case, extremely thick specimens (for example, 100 microns) require focus adjustment to see a clear, sharp image.
Image Stacking accomplishes this by capturing an image at regular focus layers for the thickness of the specimen.
The Extended Depth of Field (EDF) algorithm combines the best focused parts of several images and creates a composite image where everything is in the best focus available from the stack of individual images.
After each field image is processed by the EDF algorithm, the are stitched and combined into a large, whole scan image using the standard algorithm used by the uScope Navigator Application.
Creating a Whole Scan Image is accomplished as it is for a normal single-layer scan.
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