Information in this article applies to:
Article ID: NGN1206 — Created: 21 Aug 2019 — Reviewed: 27 Jul 2020
uScope Navigator v4.5 adds support for EDF (Extended Depth of Field) image composition. This support is composed of several processes:
These tasks are described in details below.
Multi-Layer is synonymous with Z-Stacking and Image Stacking.
Creating EDF images requires an additional software license. Please contact our sales department for more information.
Creating Multi-Layer images does not require an additional software license.
Multi-Layer Scanning (Z-Stacking or Image Stacking) involves taking several images at each scan field position at different focus points. The number of images in the stack depends on two (2) things:
Refer to Multi-Layer Focus Scan Settings for more information on the Multi-Layer Focus settings.
Multi-Layer images are created by combining the images from a single Z level into a large, whole scan image. This is enabled in the Create Whole Scan Image dialog.
EDF images are created by an Extended Depth of Field (EDF) algorithm which combines the best focused parts of each image in the Z-Stack. A composite image is created where every pixel is best focused pixel available from the stack of individual images. This is enabled by a checkbox in the Create Whole Scan Image dialog.
After each field image is (optionally) processed by the EDF algorithm, they are stitched and combined into a large, whole scan image by the uScope Navigator Application.
The whole scan images for each layer are created after the EDF whole scan image.
The uScope Navigator Deep-Zoom image viewer has been enhanced with a layer selector (focus knob) and a checkbox to select the EDF image.