Information in this article applies to:
Article ID: NSC1061 — Created: 28 Jul 2017 — Reviewed: 9 Aug 2019
After a slide is loaded into the uScope, the overview image is blank or has a strange blank spot.
This image shows the effects of flat-fielding an empty slide tray when the overview camera was calibrated with a slide remaining in the tray.
The bright white areas are a negative of the specimen on the slide that was left in the uScope during overview camera calibration. It will affect the overview image of any slide inserted into the uScope.
This image shows a completely blank image that displays when a slide was left in the unit during overview camera calibration.
This issue may be caused if the overview camera is calibrated with a slide inserted in the uScope.
When calibrating the overview camera, you are prompted to and must remove the slide so the uScope can capture a blank image to use for flat-fielding. If you accidentally leave a slide in the unit during calibration, the specimen image is used to create a flat-field image that is used to balance the displayed slide overview — and that causes the blank white areas in the overview image.
Recalibration automatically white-balances the camera and re-captures the flat-field image. Refer to the following knowledge base article to recalibrate and flat-field the objective camera.