Article ID: NSC1088
Created: 9 Nov 2017
Reviewed: 12 Nov 2017
Information in this article applies to:
When should I choose the Exhaustive Stack focus method to scan my slide?
The Exhaustive Stack focus method captures a deep stack (Z stack) of images for each field in the region of interest and chooses the one with the best focus. The best focus is determined by calculating which field has the highest contrast in the content. Generally, objects in better focused images have sharper edges which, in turn, have higher contrast to the background.
This focus method should be used when absolute best focus for each field is critical. It is the slowest focus method since it captures many images of each field in an effort to determine the best focus.
You can adjust the range and step settings for various aspects of exhaustive focus stacks in the device settings.