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Article ID: NSC1155 — Created: 23 Feb 2018 — Reviewed: 13 May 2018
What fast stack focus method settings are available and how are they used?
The Fast Stack focus method captures a short stack of images for each field in a scan. The stack is centered around the initial focus selected at the beginning of the scan.
The Fast Stack focus method is much faster than the Exhaustive Stack focus method and performs well for very flat, very thin sections. By default, the Fast Stack settings capture one image above and below the initial focus point and then compares them to determine the best focus.
The following settings control the predictive focus algorithm. You may change these parameters to suit your requirements.
The step size specifies the number of motor steps to move the objective (0.1984 microns per step). So, 10 steps are equivalent to 1.984 microns (0.001984mm). The step range specifies the range (in motor steps) above and below a mid-point. So, a step range of 20 is equivalent to 41 steps in total (20 motor steps above and 20 motor steps below the initial focus).
Refer to Step Size and Range Settings for more information.