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Article ID: NSC1202 — Created: 18 Nov 2018 — Reviewed: 18 Nov 2018
uScope Navigator v4.5 introduces a new, streamlined method for changing the settings for multi-layer scanning. Multi-layer scans are also known as Z-Stacking or Image Stacking.
Multi-layer scans involve capturing images at multiple focus layers (Z-stacking) for each field in the selected region of interest. uScope Navigator allows you to control not only the number of layers but the focus distance between each layer.
To estimate the number of layers, you first need to estimate the depth of the specimen and get the depth in focus steps. There are several ways to accomplish this:
|Objective||Depth of Field||Recommended
(Steps per Layer)
Take the specimen depth (in steps) and divide by the recommended spacing to obtain the number of layers for the multi-layer scan. Enter the spacing and number of layers in the Multi-Layer Focus Configuration box.
Select the starting position (top, middle, bottom) to correspond with the initial focus. Typically, you would select Top then set the initial scan focus to the highest point of the specimen that is in focus.
We suggest you start multi-layer scanning with a small region of interest and a small number of layers (7-13). It can take a long time to scan a region of interest with many layers.
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