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Scan Mode Creating Whole Scan Images for Multi-Layer Scans
Scan Mode

Information in this article applies to:

  • uScope Navigator V4.5

Article ID: NSC1276 — Created: 21 Aug 2019 — Reviewed: 1 Sep 2020

Summary

uScope Navigator v4.5 now supports multi-layer scanning (Z-Stacking or Image Stacking). Refer to Scanning Multi-Layer Regions (Image Stacking) for details.

Multi-Layer scans capture multiple images (at different focus levels) for each field in the scanned region of interest. A whole scan image may be created for each scanned focus layer. In addition, an EDF (extended depth-of-field) image may be created by taking the best focused data from each layer to create a composite image where everything is in focus.

Viewing these images requires a special viewer that provides access to the individual layers and composite EDF image. uScope Navigator v4.5 includes such a viewer. Refer to Viewing Multi-Layer (Image Stack) Scans for more information.

uScope Navigator v4.5 creates Whole Scan Images (Deep Zoom, SVS, and EasyZoom) for Multi-Layer scans in a single pass. Additional options have been added to the Create Whole Scan Images dialog as described below.

Details

Create Multi-Layer Whole Scan Image The Create Whole Scan Image dialog lets you choose which images to create and the options for multi-layer scans.

Output Formats

  • Deep Zoom
    Multiple Deep Zoom Images are created (one for each layer and one for the EDF). The Deep Zoom image viewer lets you view each layer/EDF.
  • SVS (Tiled TIFF)
    A separate SVS file is created for each scanned layer and EDF composite image.
  • EasyZoom (SZI)
    A separate EasyZoom file is created for each scanned layer and EDF composite image.

Multi-Layer Options

  • Create for All Layers
    Create images for each layer.
  • Create EDF
    Create a composite EDF image from all layer images.
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