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Image Viewer Viewing Multi-Layer (Image Stack) Scans
Image Viewer

Information in this article applies to:

  • uScope Navigator V4.5 and Later

Article ID: NVW1176 — Created: 21 Aug 2019 — Reviewed: 7 Dec 2020

Summary

uScope Navigator v4.5 introduces image stacking or multi-layer scanning which allows you to scan a region of interest at multiple focus points and combine them into multiple whole slide images (each on a different focal plane).

The Deep Zoom image viewer has been enhanced to provide a focus control (shown in the upper left corner of the viewer) to select the different layers of the image stack. An EDF button is present if an EDF (extended depth-of-field) composite image is available to view.

Multi-Layer Deep Zoom Viewer (Top Focus Level)
Multi-Layer Viewer (Top Focus Level)
Multi-Layer Deep Zoom Viewer (Middle Focus Level)
Multi-Layer Viewer (Middle Focus Level)
Multi-Layer Deep Zoom Viewer (Bottom Focus Level)
Multi-Layer Viewer (Bottom Focus Level)
Multi-Layer Deep Zoom Viewer (EDF Layer)
Multi-Layer Viewer (EDF Layer)

Details

The focus control simulates the effect of adjusting the focus on a traditional microscope. Moving the focus control up displays image layers that are further away from the specimen (akin to moving the objective up/away) while moving the focus control down displays image layers that are closer to the specimen (akin to moving the objective down/closer).

As you move the focus control, you'll immediately see the effects of the change in focus on the scanned region.

Note
Differences in the focus of the various layers of the scanned images may cause image stitching to combine adjacent fields in slightly different locations.

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