Using the Fast Stack Focus Method

Fast Stack Focus Method

The uScope Navigator Application offers several different focus methods you may use when scanning a region of interest. This video discusses the Fast Stack Focus Method and how it is best used.

Fast Stack is intended for use with tissue samples and other specimens that are extremely thin and flat. If your specimen is not well-prepared, this focus method will not provide acceptable focus results.

After selecting the region to scan, you are prompted to select an initial focus point. The Fast Stack Focus Method uses this focus point as the middle of a short Z-stack of images and chooses the best focused image from the stack. The fewer images that are in the stack, the faster the best focused image is acquired.

Fast Stack is a very fast focus method that works well for extremely thin sections where focus does not deviate much from field to field.

Related Videos

Using the Exhaustive Stack Focus Method

Using the Initial Focus Method

Using the Predictive Focus Method

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